The changes in the serum, liver, kidney protein and genomic DNA profiles in rats treated with 7,12-Dimethylbenz(A) anthracene and Plantago major L.
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CitationBerber, İ., Ekin, S., Levent, A., Oto, G. (2014). The changes in the serum, liver, kidney protein and genomic DNA profiles in rats treated with 7,12-Dimethylbenz(A) anthracene and Plantago major L.. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5 (1), pp. 253-260.
In the study, the changes in the serum, liver, kidney protein and genomic DNA profiles in Wistar albino rats administrated to 7,12-dimethylbenz(a)anthracene (DMBA) and Plantagomajor L. were investigated. SDS-PAGE profiling of the serum proteins showed that the levels of two proteins molecular weighing 140.8 kDa and 46.8 kDa were significantly lower on the 0th day of treatment than on the 60th day of application in the groups. Furthermore, two proteins (34.5 and 22.3 kDa) were solely present in DMBA-treated liver cell lysates. On the contrary, the kidney protein profiles did not show significant banding variations in groups. Genomic DNA analysis also confirmed that DNA extracted from liver and kidney cells did not fragment in any of the groups after the 60th study day. The proteins 34.5 and 22.3 kDa in liver cell lysates at the 60th day in DMBA-treated group could be extra-prognostic indicators for severe DMBA toxicity.