Electrochemical oxidation of vildagliptin on pencil graphite electrode: Extremely sensitive determination in drugs and human urine with square wave voltammetry

Abstract

Vildagliptin (VLDG), one of the antidiabetic agents, is a dipeptidyl peptidase 4 inhibitor. It is a drug developed for oral administration[1]. In the literature, there is only one study in which electrochemical methods were used for the analysis of Vildagliptin in biological fluid and drug forms[2]. In this study, the electrochemical properties of VLDG, which is one of the drugs used as an antidiabetic agent, were determined by using pencil graphite electrode in phosphate buffer solution (PBS/pH 9.0). The irreversible and diffusion controlled adsorption oxidation peak was measured by cyclic voltammetry at approximately +1.13 V for this compound. Using square wave stripping voltammetry, the current showed a linear correlation in PBS buffer at pH 9.0, with a concentration range of 2.94 to 49.98 µM. At a concentration of 2.94 µM (n=9), the limit of detection of 8.20 nM (2.48 ng mL-1 ) and a relative standard deviation of 2.95 % were calculated. This method was successfully applied for VLDG analysis without any separation in pharmaceutical preparations and urine samples