Aral, HayriyeHaşimi, DuyguAral, TarıkLevent, AbdulkadirZiyadanoğulları, Berrin2019-06-212019-06-212017-07-06Aral, H., Haşimi, D., Aral, T., Levent, A., Ziyadanoğullari, B. (2017). Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase. Journal of Liquid Chromatography & Related Technologies, 40(11), pp. 549-555. https://doi.org/10.1080/10826076.2017.13330051082-60761520-572Xhttps://doi.org/10.1080/10826076.2017.1333005https://hdl.handle.net/20.500.12402/2098In this study, some plant growth regulators known as cytokinins [kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03–0.1, 0.1–0.26 mg/L, and 0.03–0.08, respectively.eninfo:eu-repo/semantics/closedAccessAttribution-NonCommercial-ShareAlike 3.0 United StatesAmide-Embedded Stationary PhaseCytokininsMixed-Mode Stationary PhasePlant Growth RegulatorsSalvia LimbataArticle4011549555Q3Q4