Determination of 7,12-dimethylbenz[a]anthracene in orally treated rats by high-performance liquid chromatography and transfer stripping voltammetry

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dc.authorid0000-0002-9587-096Xen_US
dc.authorid0000-0001-5792-419Xen_US
dc.authorid0000-0002-6502-5028en_US
dc.authorid0000-0001-5216-3520en_US
dc.authorid0000-0001-7310-7800en_US
dc.authorid0000-0002-0356-9345en_US
dc.contributor.authorYardım, Yavuz
dc.contributor.authorLevent, Abdulkadir
dc.contributor.authorEkin, Suat
dc.contributor.authorKeskin, Ertuğrul
dc.contributor.authorOto, Gökhan
dc.contributor.authorŞentürk, Zühre
dc.date.accessioned2021-03-23T11:52:27Z
dc.date.available2021-03-23T11:52:27Z
dc.date.issued2012-06en_US
dc.departmentBatman Üniversitesi Fen - Edebiyat Fakültesi Kimya Bölümüen_US
dc.description.abstractA number of polycyclic aromatic hydrocarbons (PAHs) have been shown to be toxicants, and induce carcinogenic and immunotoxic effects. As a model PAH agent, 7,12-dimethylbenz[a]anthracene (DMBA) was the strongest one tested in terms of its biological activities and biotransformation. A new and simple high-performance liquid chromatographic (HPLC) method with diode-array detection at 290 nm was developed and validated for monitoring of DMBA in different matrices (serum, liver and kidney) of rats orally treated with DMBA. Furthermore, the applicability of adsorptive transfer stripping voltammetry (AdTSV) on the pencil-lead graphite electrode to these samples was illustrated using our previously reported data for bulk aqueous solutions of DMBA. HPLC and AdTSV methods, which were compatible with each other, allowed DMBA to be detected down to the levels of 3.82x10-9 M (0.98 ppb) and 6.73x10-9 M (1.73 ppb), respectively. Olive oil solutions of DMBA in dose 50 mg/kg were orally administered. 60 days after a single dose of DMBA, its concentrations in these biological samples from rats were measured by means of both methods. Because of rapid biotransformation, DMBA could not be detected in serum. Only low levels of the compounds were deposited unchanged in kidney whereas its levels were considerably higher in liver. These methods were also applied to the assay whether there is an influence of the intake of aqueous extracts of Hypericum Perforatum L. plant on the parent DMBA levels accumulated in rat tissues.en_US
dc.identifier.citationYardım, Y., Levent, A., Ekin, S., Keskin, E., Oto, G., Şentürk, Z. (2012). Determination of 7,12-dimethylbenz[a]anthracene in orally treated rats by high-performance liquid chromatography and transfer stripping voltammetry. Combinatorial Chemistry and High Throughput Screening, 15 (5), pp. 418-426. https://dx.doi.org/10.2174/138620712800194440.en_US
dc.identifier.endpage426en_US
dc.identifier.issn1386-2073
dc.identifier.issn1875-5402
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage418en_US
dc.identifier.urihttps://dx.doi.org/10.2174/138620712800194440
dc.identifier.urihttps://hdl.handle.net/20.500.12402/2772
dc.identifier.volume15en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBenthamen_US
dc.relation.isversionof10.2174/138620712800194440en_US
dc.relation.journalCombinatorial Chemistry and High Throughput Screeningen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject7,12-Dimethylbenz[a]anthracene (DMBA) Determinationen_US
dc.subjectAdsorptive Transfer Stripping Voltammetryen_US
dc.subjectHighperformance Lliquid Chromatographyen_US
dc.subjectHypericum Perforatum Len_US
dc.subjectRat.en_US
dc.titleDetermination of 7,12-dimethylbenz[a]anthracene in orally treated rats by high-performance liquid chromatography and transfer stripping voltammetryen_US
dc.typeArticleen_US

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