Synthesis, characterization and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography

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dc.authorid0000-0002-6612-2751en_US
dc.contributor.authorAral, Tarık
dc.contributor.authorAral, Hayriye
dc.contributor.authorÇelik, Kadir Serdar
dc.contributor.authorAltındağ, Ramazan
dc.date.accessioned2021-03-24T07:56:30Z
dc.date.available2021-03-24T07:56:30Z
dc.date.issued2017-11-1en_US
dc.departmentBatman Üniversitesi Fen - Edebiyat Fakültesi Kimya Bölümüen_US
dc.description.abstractA novel multifunctional stationary phase based on silica gel was synthesised starting from L- isoleucine and 4-phenylbutylamine and evaluated as a hydrophilic interaction/reversed-phase mixed-mode stationary phase for high-performance liquid chromatography (HPLC). The prepared stationary phase was characterized by elemental analysis, infrared spectroscopy (IR), scanning electron microscopy (SEM), Brunauer, Emmett and Teller (BET) and solid-state 13C nuclear magnetic resonance (NMR). The mechanisms involved in the chromatographic separation are multi-interaction, including hydrophobic, π-π, hydrogen-bonding, dipole-dipole and ion-dipole interactions. Based on these interactions, successful separation could be achieved among several aromatic compounds having different polarities under both hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) condition. Nucleotides/nucleosides were separated in the HILIC mode. The effects of different separation conditions, such as pH value, mobile-phase content, column temperature, buffer concentration and flow rate, on the separation of nucleotides/nucleosides in HILIC mode were investigated. The seven nucleotides/nucleosides were separated within 22 min, while six of them were separated within 10 min by isocratic elution. To determine the influence of the new multifunctional stationary phase under the RP condition, a number of moderately and weakly polar and nonpolar compounds, such as 10 substituted anilines and eight substituted phenols were separated successfully under the RP condition within 14 and 15 min, respectively. Additionally, nine mixtures of polar/nonpolar test compounds were simultaneously separated within 19 min, while seven of them were separated within 12 min, under HILIC/RP mixed-mode conditions. Chromatographic parameters, such as the retention factor and peak asymmetry factor, were calculated for all of the analytes, while the theoretical plate number was calculated for analytes separated by isocratic elution. Compared to traditional C18 and commercial HILIC columns, the new stationary phase exhibited both HILIC and RPLC performance, and the scope of analyte separation was thus enlarged.en_US
dc.identifier.citationAral, T., Aral, H., Çelik, K. S., Altındağ, R. (2017). Synthesis, characterization and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography. Talanta. 174, pp.703-714. https://doi.org/10.1016/j.talanta.2017.07.014en_US
dc.identifier.endpage714en_US
dc.identifier.issn0039-9140
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage703en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2017.07.014
dc.identifier.urihttps://hdl.handle.net/20.500.12402/2781
dc.identifier.volume174en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTalantaen_US
dc.relation.isversionof10.1016/j.talanta.2017.07.014en_US
dc.relation.journalTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectMixed-Mode Chromatographyen_US
dc.subjectMixed-Mode Stationary Phaseen_US
dc.subjectPhase Hydrophilic İnteraction Liquid Chromatography (HILIC)en_US
dc.subjectReversed Phase Liquid Chromatographyen_US
dc.titleSynthesis, characterization and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatographyen_US
dc.typeArticleen_US

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