Method validation and simultaneous determination of retinol, retinyl palmitate, β-Carotene, α-Tocopherol and vitamin C in Rat serum treated with 7,12 dimethylbenz[a]anthracene and plantago major L. By high- Performance liquid chromatography using diode-array detection

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dc.authorid0000-0001-5792-419Xen_US
dc.authorid0000-0001-7310-7800en_US
dc.authorid0000-0002-6502-5028en_US
dc.contributor.authorLevent, Abdulkadir
dc.contributor.authorOto, Gökhan
dc.contributor.authorEkin, Suat
dc.contributor.authorBerber, İsmet
dc.date.accessioned2021-03-23T11:01:32Z
dc.date.available2021-03-23T11:01:32Z
dc.date.issued2013-02en_US
dc.departmentBatman Üniversitesi Fen - Edebiyat Fakültesi Kimya Bölümüen_US
dc.description.abstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a]anthracene. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-tetrahydrofuran-water as mobile phase under gradient conditions, at 1.5 mL min-1 flow rate and 25 °C. Diode-array detection was at 325, 450, 290 and 270 nm (retinol and retinyl palmitate), β-carotene, α-tocopherol and vitamin C, respectively and runnig time 18 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum. Results of this study demonstrated that; at 60th day DMBA-treated group, there was a significant decrease in vitamin levels compared to the levels of control group. A significant increase was observed in vitamin levels of 7,12 dimethylbenz[a]anthracene+Plantago Major L.-treated group compared to the DMBA-treated group. Additionally, the results obtained in the study are found to be in agreement with data reported in the literature.en_US
dc.identifier.citationLevent, A., Oto, G., Ekin, S., Berber, İ. (2013). Method validation and simultaneous determination of retinol, retinyl palmitate, β-Carotene, α-Tocopherol and vitamin C in Rat serum treated with 7,12 dimethylbenz[a]anthracene and plantago major L. By high- Performance liquid chromatography using diode-array detection. https://doi.org/10.2174/1386207311316020008.en_US
dc.identifier.endpage149en_US
dc.identifier.issn1386-2073
dc.identifier.issn1875-5402
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage142en_US
dc.identifier.urihttps://doi.org/10.2174/1386207311316020008
dc.identifier.urihttps://hdl.handle.net/20.500.12402/2768
dc.identifier.volume16en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBenthamen_US
dc.relation.isversionof10.2174/1386207311316020008en_US
dc.relation.journalCombinatorial Chemistry and High Throughput Screeningen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject12 Dimethylbenz[a]anthraceneen_US
dc.subjectHPLCen_US
dc.subjectPlantago Major L. 7en_US
dc.subjectSerumen_US
dc.subjectVitaminen_US
dc.titleMethod validation and simultaneous determination of retinol, retinyl palmitate, β-Carotene, α-Tocopherol and vitamin C in Rat serum treated with 7,12 dimethylbenz[a]anthracene and plantago major L. By high- Performance liquid chromatography using diode-array detectionen_US
dc.typeArticleen_US

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