Scopus İndeksli Yayınlar Koleksiyonu

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https://hdl.handle.net/20.500.12402/1912

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“Balcı” aspir (Carthamus tinctorius l.) çeşidinin in vitro sürgün uçlarının mikroçoğaltımı ve köklendirilmesi üzerine oksin ve sitokininlerin etkisi
(Yüzüncü Yıl Üniversitesi, 2018-12-31) Akbaş, Filiz; Hamidi Birecikli, Atike
Bu çalışmada, linoleik tipte olan Balcı aspir çeşidinin, mikroçoğaltımı ve köklendirilmesi üzerine bazı sitokinin (BAP ve Kin) ve oksinlerin (NAA ve IAA) etkisi incelenerek in vitro çoğaltım protokolünün geliştirilmesi amaçlanmıştır. Başlangıç materyali olarak, in vitro koşullarda çimlendirilen olgun tohumlardan elde edilen steril fidelerin sürgün uçları kullanılmıştır. Sürgün uçları mikroçoğaltım için, BAP ve Kin’in farklı konsantrasyonlarının (0.0, 0.5, 1.0, 2.0 ve 4.0 mg/L) bulunduğu MS besi ortamında kültüre alınmıştır. Elde edilen sürgünler köklendirme aşamasında farklı NAA ve IAA (0.0, 0.5, 1.0 ve 2.0 mg/L) konsantrasyonlarını içeren MS besi ortamına transfer edilmiştir. BAP konsantrasyonları arasında en iyi sürgün çoğaltımı, eksplant başına 5.33 adet sürgün ile 0.5 mg/L BAP’lı ortamdan elde edilirken, Kin uygulamalarında ise 3.75 adet sürgün ile 0.5 mg/L Kin ve 3.33 adet sürgün ile 4.0 mg/L Kin içeren ortamdan elde edilmiştir. Ancak BAP uygulamalarının genelinde ve 4.0 mg/L Kin uygulamasında oluşan sürgünlerde vitrifikasyon olduğu belirlenmiştir. Bu nedenle, 0.5 mg/L Kin ile desteklenmiş MS besi ortamının Balcı aspir çeşidinin in vitro sürgün çoğaltımı için en ideal ortam olduğu tespit edilmiştir. En iyi kök oluşumunun, eksplant başına 20 adet ile 2.0 mg/L NAA içeren ortamda kültüre alınan sürgünlerde olduğu saptanmıştır. Elde edilen köklü fideler torf – perlit karışımı içeren saksılara dikilerek toprağa adaptasyonu sağlanmıştır.
Callus induction and plant regeneration from different explants of actinidia deliciosa
(Springer Nature, 2008-10-31) Akbaş, Filiz; Işıkalan, Çiğdem; Namlı, Süreyya
In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M1, 1.0 mg l-1 BA + 2.0 mg l-1 2,4-D-M 2, 1.0 mg l-1 NAA + 2.0 mg l-1 2,4-D) on initiation of callus were examined in order to obtain callus for organogenesis. The best callus for plant regeneration was obtained from leaf explants on Murashige and Skoog's medium (MS) supplemented with M2. Formation of callus from leaf of kiwifruit (A. deliciosa) was cultured in MS medium containing different concentration of N6-benzylaminopurin (BA; 0.0, 1.0, 2.0, 4.0, 6.0, 8.0 mg l-1) for callus proliferation and plant regeneration. Although the first shoot formation was appeared in medium containing 6.0 and 8.0 mg l-1 BA, the best shoots formation was obtained in medium with 4.0 mg l-1 BA.
A detailed chemical and biological investigation of twelve allium species from Eastern Anatolia with chemometric studies
(Wiley-Blackwell, 2020-11-14) İzol, Ebubekir; Temel, Hamdi; Yılmaz, Mustafa Abdullah; Yener, İsmail; Tokul Ölmez, Özge; Kaplaner, Erhan; Fırat, Mehmet; Haşimi, Nesrin; Öztürk, Mehmet; Ertaş, Abdulselam
Allium species are widely consumed as food all over the world. The phenolic profile of ethanol extracts of aerial parts and roots of 12 Allium species, collected from five different Eastern Anatolia regions, were studied using LC-MS/MS. In vitro antioxidant, anticholinesterase, cytotoxic and antimicrobial activities were also tested. The multivariate analyses were performed using principal component and hierarchical cluster analyses. Seventeen of 27 standard compounds were detected in all Allium species. The major components were mainly identified as quinic acid, malic acid, vanillin, and p-coumaric acid. The aerial parts possessed better antioxidant activity than roots. Aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense exhibited high cytotoxic activity against DLD-1 colon cancer cell lines (IC50 12.5 μg/mL). A. shatakiense and A. vineale demonstrated good antimicrobial activity against S. aureus and E. coli (MIC 75 μg/mL). According to chemometric analysis, differences were detected between aerial parts and the roots. The aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense could be potent in the pharmaceutical industry while A. shatakiense and A. vineale in the food industry after further investigations.
Karyotypes of the Mole Rats, genus Nannospalax (Palmer, 1903) (Spalacidae: Rodentia) populations in eastern Anatolia, Turkey
(Research Affairs of Ferdowsi University of Mashhad, 2012) Coşkun, Yüksel; Kaya, Alaettin; Ulutürk, Servet; Yürümez, Gökhan; Moradi, Mohammad
The Spalacidae are Southeast European and East Mediterranean blind rodents, highly adapted for life underground. Their taxonomy needs a modern revision including chromosomal data as well as morphology. Mole rats of the family Spalacidae range over Turkey and approximately 30 karyotypes of Nannospalax complex inhabit. The diploid number of chromosome of Nannospalax ranges from 36 to 62. Also, fundamental number of chromosomal arms, NF values vary from 66 to 92 while the fundamental number of autosomal arms, NFa ranging from 62 to 88. Unfortunely, karyological studies of the Nannospalax populations on the territory of Turkey on the whole are far from being satisfactory. Karyological studies of this group may yield further chromosomal forms in Turkey, which has a wide range of climatic and biotic conditions and the boundaries of the distribution region of the known species might be determined. In this study, chromosomal forms of Nannospalax in East Anatolia (Erzurum, Kars and Ağrı province) were investigated. The materials of 16 specimens of Nannospalax were collected at 8 different localities in the region. Preparations of mitotic chromosomes were obtained from bone marrows by means of the general air-drying technique. Skins and skulls of specimens have been deposited at the Dicle University, Science Faculty Biology Department. We have identified two chromosomal forms of Nannospalax in East Anatolia of which have diploid chromosome numbers (2n) are 2n = 48 and 2n = 50

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