Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones
Yükleniyor...
Tarih
2013-11
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-ShareAlike 3.0 United States
Attribution-NonCommercial-ShareAlike 3.0 United States
Özet
A novel amide-bonded silica stationary phase was prepared starting from N-Boc-phenylalanine, cyclohexylamine and spherical silica gel (4 μm, 60 Å). The amide ligand was synthesised with high yield. The resulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. The resulting selector bearing a polar amide group is used for the reversed-phase chromatography separation of different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours of these analytes on the amide-silica stationary phase were compared with those of RP-C18 column under same conditions. The effects of different separation conditions, such as mobile phase, pH value, flow rate and temperature, on the separation and retention behaviours of the 13 phytohormones in this system were studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with an aqueous mobile phase containing pH=6.85 potassium phosphate buffer (20 mM) and acetonitrile with a 22 C column temperature. Under these experimental conditions, the 12 phytohormones could be separated and detected at 230 or 270 nm within 26 min.
Açıklama
Anahtar Kelimeler
Amide Embedded, Plant Growth Regulators, Amide Stationary Phase, Herbicides, HPLC Separation, Reversed-Phase
Kaynak
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
116
Sayı
Künye
Aral, H., Aral, T., Ziyadanoğulları, B., Ziyadanoğulları, R. (2013). Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones. Talanta, 116, pp. 155-163. https://doi.org/10.1016/j.talanta.2013.05.007
