Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones

Basit Öğe Kaydı
dc.authorid0000-0001-7811-8816en_US
dc.contributor.authorAral, Hayriye
dc.contributor.authorAral, Tarık
dc.contributor.authorZiyadanoğulları, Berrin
dc.contributor.authorZiyadanoğulları, Recep
dc.date.accessioned2019-06-21T12:13:06Z
dc.date.available2019-06-21T12:13:06Z
dc.date.issued2013-11en_US
dc.departmentBatman Üniversitesi Fen - Edebiyat Fakültesi Kimya Bölümüen_US
dc.description.abstractA novel amide-bonded silica stationary phase was prepared starting from N-Boc-phenylalanine, cyclohexylamine and spherical silica gel (4 μm, 60 Å). The amide ligand was synthesised with high yield. The resulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. The resulting selector bearing a polar amide group is used for the reversed-phase chromatography separation of different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours of these analytes on the amide-silica stationary phase were compared with those of RP-C18 column under same conditions. The effects of different separation conditions, such as mobile phase, pH value, flow rate and temperature, on the separation and retention behaviours of the 13 phytohormones in this system were studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with an aqueous mobile phase containing pH=6.85 potassium phosphate buffer (20 mM) and acetonitrile with a 22 C column temperature. Under these experimental conditions, the 12 phytohormones could be separated and detected at 230 or 270 nm within 26 min.en_US
dc.identifier.citationAral, H., Aral, T., Ziyadanoğulları, B., Ziyadanoğulları, R. (2013). Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones. Talanta, 116, pp. 155-163. https://doi.org/10.1016/j.talanta.2013.05.007en_US
dc.identifier.endpage163en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage155en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2013.05.007
dc.identifier.urihttps://hdl.handle.net/20.500.12402/2103
dc.identifier.volume116en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.talanta.2013.05.007en_US
dc.relation.journalTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectAmide Embeddeden_US
dc.subjectPlant Growth Regulatorsen_US
dc.subjectAmide Stationary Phaseen_US
dc.subjectHerbicidesen_US
dc.subjectHPLC Separationen_US
dc.subjectReversed-Phaseen_US
dc.titleDevelopment of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormonesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
1-s2.0-S0039914013004050-main.pdf
Boyut:
1.57 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Fen - Edebiyat Fakültesi, Kimya Bölümü, Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu