Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase

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Küçük Resim

Tarih

2017-07-06

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Taylor & Francis

Erişim Hakkı

info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-ShareAlike 3.0 United States

Özet

In this study, some plant growth regulators known as cytokinins [kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03–0.1, 0.1–0.26 mg/L, and 0.03–0.08, respectively.

Açıklama

Anahtar Kelimeler

Amide-Embedded Stationary Phase, Cytokinins, Mixed-Mode Stationary Phase, Plant Growth Regulators, Salvia Limbata

Kaynak

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

40

Sayı

11

Künye

Aral, H., Haşimi, D., Aral, T., Levent, A., Ziyadanoğullari, B. (2017). Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase. Journal of Liquid Chromatography & Related Technologies, 40(11), pp. 549-555. https://doi.org/10.1080/10826076.2017.1333005