Development of a novel amide silica stationary phase for the reversed phase HPLC separation of different classes of phytohormones
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Dosyalar
Tarih
2013-05-14
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Journals & Books
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info:eu-repo/semantics/restrictedAccess
Attribution-NonCommercial-ShareAlike 3.0 United States
Attribution-NonCommercial-ShareAlike 3.0 United States
Özet
A novel amide-bonded silica stationary phase was prepared starting fromN-Boc-phenylalanine, cyclo-hexylamine and spherical silica gel (4mm, 60 Å). The amide ligand was synthesised with high yield. Theresulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. Theresulting selector bearing a polar amide group is used for the reversed-phase chromatography separationof different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours ofthese analytes on the amide-silica stationary phase were compared with those of RP-C18 column undersame conditions. The effects of different separation conditions, such as mobile phase, pH value,flow rateand temperature, on the separation and retention behaviours of the 13 phytohormones in this systemwere studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with anaqueous mobile phase containing pH¼6.85 potassium phosphate buffer (20 mM) and acetonitrile with a221C column temperature. Under these experimental conditions, the 12 phytohormones could beseparated and detected at 230 or 270 nm within 26 min
A novel amide-bonded silica stationary phase was prepared starting fromN-Boc-phenylalanine, cyclo-hexylamine and spherical silica gel (4mm, 60 Å). The amide ligand was synthesised with high yield. Theresulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. Theresulting selector bearing a polar amide group is used for the reversed-phase chromatography separationof different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours ofthese analytes on the amide-silica stationary phase were compared with those of RP-C18 column undersame conditions. The effects of different separation conditions, such as mobile phase, pH value,flow rateand temperature, on the separation and retention behaviours of the 13 phytohormones in this systemwere studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with anaqueous mobile phase containing pH¼6.85 potassium phosphate buffer (20 mM) and acetonitrile with a221C column temperature. Under these experimental conditions, the 12 phytohormones could beseparated and detected at 230 or 270 nm within 26 min
A novel amide-bonded silica stationary phase was prepared starting fromN-Boc-phenylalanine, cyclo-hexylamine and spherical silica gel (4mm, 60 Å). The amide ligand was synthesised with high yield. Theresulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. Theresulting selector bearing a polar amide group is used for the reversed-phase chromatography separationof different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours ofthese analytes on the amide-silica stationary phase were compared with those of RP-C18 column undersame conditions. The effects of different separation conditions, such as mobile phase, pH value,flow rateand temperature, on the separation and retention behaviours of the 13 phytohormones in this systemwere studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with anaqueous mobile phase containing pH¼6.85 potassium phosphate buffer (20 mM) and acetonitrile with a221C column temperature. Under these experimental conditions, the 12 phytohormones could beseparated and detected at 230 or 270 nm within 26 min
Açıklama
Anahtar Kelimeler
Kaynak
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
116
Sayı
Künye
Aral, T., Aral, H., Ziyadanoğulları, B., Ziyadanoğulları, R. (2013). Development of a novel amide silica stationary phase for the reversed phase HPLC separation of different classes of phytohormones. Talanta 116, pp.155-163.
