Development of a novel amide silica stationary phase for the reversed phase HPLC separation of different classes of phytohormones

Basit Öğe Kaydı
dc.authorid0000-0002-6612-2751en_US
dc.contributor.authorAral, Tarık
dc.contributor.authorAral, Hayriye
dc.contributor.authorZiyadanoğulları, Berrin
dc.contributor.authorZiyadanoğulları, Recep
dc.date.accessioned2021-03-24T11:55:30Z
dc.date.available2021-03-24T11:55:30Z
dc.date.issued2013-05-14en_US
dc.departmentBatman Üniversitesi Fen - Edebiyat Fakültesi Kimya Bölümüen_US
dc.description.abstractA novel amide-bonded silica stationary phase was prepared starting fromN-Boc-phenylalanine, cyclo-hexylamine and spherical silica gel (4mm, 60 Å). The amide ligand was synthesised with high yield. Theresulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. Theresulting selector bearing a polar amide group is used for the reversed-phase chromatography separationof different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours ofthese analytes on the amide-silica stationary phase were compared with those of RP-C18 column undersame conditions. The effects of different separation conditions, such as mobile phase, pH value,flow rateand temperature, on the separation and retention behaviours of the 13 phytohormones in this systemwere studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with anaqueous mobile phase containing pH¼6.85 potassium phosphate buffer (20 mM) and acetonitrile with a221C column temperature. Under these experimental conditions, the 12 phytohormones could beseparated and detected at 230 or 270 nm within 26 minen_US
dc.description.abstractA novel amide-bonded silica stationary phase was prepared starting fromN-Boc-phenylalanine, cyclo-hexylamine and spherical silica gel (4mm, 60 Å). The amide ligand was synthesised with high yield. Theresulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. Theresulting selector bearing a polar amide group is used for the reversed-phase chromatography separationof different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours ofthese analytes on the amide-silica stationary phase were compared with those of RP-C18 column undersame conditions. The effects of different separation conditions, such as mobile phase, pH value,flow rateand temperature, on the separation and retention behaviours of the 13 phytohormones in this systemwere studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with anaqueous mobile phase containing pH¼6.85 potassium phosphate buffer (20 mM) and acetonitrile with a221C column temperature. Under these experimental conditions, the 12 phytohormones could beseparated and detected at 230 or 270 nm within 26 minen_US
dc.identifier.citationAral, T., Aral, H., Ziyadanoğulları, B., Ziyadanoğulları, R. (2013). Development of a novel amide silica stationary phase for the reversed phase HPLC separation of different classes of phytohormones. Talanta 116, pp.155-163.en_US
dc.identifier.endpage163en_US
dc.identifier.issn0039-9140
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage155en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12402/2791
dc.identifier.volume116en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherJournals & Booksen_US
dc.relation.journalTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleDevelopment of a novel amide silica stationary phase for the reversed phase HPLC separation of different classes of phytohormonesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
aral.pdf
Boyut:
1.57 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Fen - Edebiyat Fakültesi, Kimya Bölümü, Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu