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Öğe A detailed chemical and biological investigation of twelve allium species from Eastern Anatolia with chemometric studies(Wiley-Blackwell, 2020-11-14) İzol, Ebubekir; Temel, Hamdi; Yılmaz, Mustafa Abdullah; Yener, İsmail; Tokul Ölmez, Özge; Kaplaner, Erhan; Fırat, Mehmet; Haşimi, Nesrin; Öztürk, Mehmet; Ertaş, AbdulselamAllium species are widely consumed as food all over the world. The phenolic profile of ethanol extracts of aerial parts and roots of 12 Allium species, collected from five different Eastern Anatolia regions, were studied using LC-MS/MS. In vitro antioxidant, anticholinesterase, cytotoxic and antimicrobial activities were also tested. The multivariate analyses were performed using principal component and hierarchical cluster analyses. Seventeen of 27 standard compounds were detected in all Allium species. The major components were mainly identified as quinic acid, malic acid, vanillin, and p-coumaric acid. The aerial parts possessed better antioxidant activity than roots. Aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense exhibited high cytotoxic activity against DLD-1 colon cancer cell lines (IC50 12.5 μg/mL). A. shatakiense and A. vineale demonstrated good antimicrobial activity against S. aureus and E. coli (MIC 75 μg/mL). According to chemometric analysis, differences were detected between aerial parts and the roots. The aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense could be potent in the pharmaceutical industry while A. shatakiense and A. vineale in the food industry after further investigations.Öğe The evaluation of total phenolic, flavonoid, sugar contents and antioxidant activity of tayfi grape in Turkey(Batman Üniversitesi, 2017) Kuru, İbrahim Selçuk; Karakuş Orcan, Pınar; Akbaş, Filiz; Işıkalan, Çiğdem; Namlı, SüreyyaTayfi (Vitis vinifera L.) only grown in the Southeastern Anatolia Region provides a major contribution on the economy of the region people. But, the research related to nutritional content of this species was not found. This study was carried out to determine the total phenolic, flavonoids, sugar content and antioxidant activity of different organe (mature/young seed and leaf ) ‘Tayfi’ grape. The highest amount of total phenolic (380,94 μg GAE/mg extract) was found to be in the young seeds. The high amount of total flavonoid content (107,21 µg QEs/mg extract) was obtained from young leaves. Higher antioxidant activity was observed in young seeds (91,32%) as expected from high contents of total phenolics. In general, as the total phenolics in seed increased, the antioxidant activities also increased. Also, changes in sugar content (glucose, fructose, sucrose and maltose) were determined in leaf from seed (young and mature) of Tayfi grape by HPLC. The highest concentration of sucrose was descripted in mature seed (9,29 mg/g).
