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Öğe Karyotypes of some small mammals inhabiting the province of Diyarbakır Turkey(Mosul University, 2011) Ulutürk, Servet; Coşkun, Yüksel; El Naemy, A; Kaya, AlaettinÖğe Karyotypes of the Mole Rats, genus Nannospalax (Palmer, 1903) (Spalacidae: Rodentia) populations in eastern Anatolia, Turkey(2012) Coşkun, Yüksel; Kaya, Alaettin; Ulutürk, Servet; Moradi, Mohammad; Yürümez, GökhanÖğe Callus induction and plant regeneration from different explants of actinidia deliciosa(Springer Nature, 2008-10-31) Akbaş, Filiz; Işıkalan, Çiğdem; Namlı, SüreyyaIn this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M1, 1.0 mg l-1 BA + 2.0 mg l-1 2,4-D-M 2, 1.0 mg l-1 NAA + 2.0 mg l-1 2,4-D) on initiation of callus were examined in order to obtain callus for organogenesis. The best callus for plant regeneration was obtained from leaf explants on Murashige and Skoog's medium (MS) supplemented with M2. Formation of callus from leaf of kiwifruit (A. deliciosa) was cultured in MS medium containing different concentration of N6-benzylaminopurin (BA; 0.0, 1.0, 2.0, 4.0, 6.0, 8.0 mg l-1) for callus proliferation and plant regeneration. Although the first shoot formation was appeared in medium containing 6.0 and 8.0 mg l-1 BA, the best shoots formation was obtained in medium with 4.0 mg l-1 BA.Öğe Yeni izole edilen bacillus licheniformis KG9’dan β- galaktozidazın üretimi, kısmi saflaştırılması ve karakterizasyonu(Batman Üniversitesi, 2013) Kaplan, Alevcan; Gül Güven, Reyhan; Güven, KemalBatman’da (Taşlıdere, Türkiye) kaplıca sularından izole edilen, Bacillus licheniformis KG9 yeni bir ekstrasellüler β-galaktozidazın üretimi için büyük bir potansiyele sahiptir. Kültür koşulları ve ortam optimizasyonu NB (Nutrienth broth) β- galaktozidaz enziminin üretimi için gerekli olduğunu ortaya çıkarmıştır. Termostabil ekstrasellüler bir enzim olan β-galaktozidaz amonyum sülfat çöktürmesi ve diyaliz ile kısmen saflaştırılmıştır. Enzimin spesifik aktivitesi 1631 U/mg proteinden 19030.45 U/mg proteine yükseltilmiştir. Kısmi saflaştırılmış enzimin moleküler ağırlığının nondenatüre poliakrilamid jel elektroforezi ile 116 kDa’dan az olduğu tespit edilmiştir. Laktoz içeren ve içermeyen ortamlarda, çalkalama şişelerinde büyümenin daha sonraki aşamalarında (96 saat) enzim aktivitesinde önemli bir üretim seviyesi belirlenmiştir, bunlar sırasıyla (2.45 U/mg protein) ve (3.25 U/mg protein)’dir. Enzim için optimum pH ve sıcaklık 8.0 ile 55 °C’dir. Kullanılan çeşitli karbon ve azot kaynakları arasında, karbon kaynaklarının enzim üretimini arttırmadığı ancak laktozun araştırılan diğer karbon kaynaklarından daha yüksek aktivite gösterdiği belirlenmiştir oysa, azot kaynaklarından glisin ve amonyum sülfat enzim üretimini hafif bir şekilde yükseltmiştir.Öğe A detailed chemical and biological investigation of twelve allium species from Eastern Anatolia with chemometric studies(Wiley-Blackwell, 2020-11-14) İzol, Ebubekir; Temel, Hamdi; Yılmaz, Mustafa Abdullah; Yener, İsmail; Tokul Ölmez, Özge; Kaplaner, Erhan; Fırat, Mehmet; Haşimi, Nesrin; Öztürk, Mehmet; Ertaş, AbdulselamAllium species are widely consumed as food all over the world. The phenolic profile of ethanol extracts of aerial parts and roots of 12 Allium species, collected from five different Eastern Anatolia regions, were studied using LC-MS/MS. In vitro antioxidant, anticholinesterase, cytotoxic and antimicrobial activities were also tested. The multivariate analyses were performed using principal component and hierarchical cluster analyses. Seventeen of 27 standard compounds were detected in all Allium species. The major components were mainly identified as quinic acid, malic acid, vanillin, and p-coumaric acid. The aerial parts possessed better antioxidant activity than roots. Aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense exhibited high cytotoxic activity against DLD-1 colon cancer cell lines (IC50 12.5 μg/mL). A. shatakiense and A. vineale demonstrated good antimicrobial activity against S. aureus and E. coli (MIC 75 μg/mL). According to chemometric analysis, differences were detected between aerial parts and the roots. The aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense could be potent in the pharmaceutical industry while A. shatakiense and A. vineale in the food industry after further investigations.Öğe Distribution of indian crested porcupine hystrix indica (Kerr, 1792) (Mammalia: Rodentia) in Batman province(Batman Üniversitesi, 2016) Yürümez, Gökhan; Ulutürk, ServetIn this study, it is aimed to detect the statue of Hystrix indica (Kerr, 1792) in Batman province. H. indica (Indian crested porcupine) is placed under the category of “least concern” (LC) species in the IUCN Red List, however it is a near threatened (NT) rodent species in Turkey. Three Indian crested porcupines were observed in three different localities in Batman province. The bio-ecological properties and protection measures of H. indica, which was investigated by direct and indirect observation techniques between the years of 2014 and 2015 in the province of Batman, were recorded in this study.Öğe Geometric morphometrics comparation of two chromosomal forms (2n=52 and 56) of nannospalax ehrenbergi (Nehring 1898) from Southeast Region in Turkey(Batman Üniversitesi, 2016) Yürümez, Gökhan; Coşkun, YükselIn this study, 26 (13 ♀♀, 13 ♂♂) specimens of two chromosomal forms of Nannospalax ehrenbergi from Mardin and Siirt-Batman provinces were compared with using by geometric morphometrics to evaluate by taxonomically. According to the results of Relative Warp Analysis (RWA), Principal Component Analysis (PCA) and Canonical Variance Analysis (CVA), populations of 2n=52 (Mardin) and 2n=56 (Siirt-Batman) are different from each other by geometric morphometrics. Also according to the CVA, female and male populations’ skulls of 2n=56 (Siirt-Batman) showed that sexual dimorphism. Skulls of specimens are deposited at the Biology Department, Science Faculty, Dicle University, TurkeyÖğe Karyotypes of the Mole Rats, genus Nannospalax (Palmer, 1903) (Spalacidae: Rodentia) populations in eastern Anatolia, Turkey(Research Affairs of Ferdowsi University of Mashhad, 2012) Coşkun, Yüksel; Kaya, Alaettin; Ulutürk, Servet; Yürümez, Gökhan; Moradi, MohammadThe Spalacidae are Southeast European and East Mediterranean blind rodents, highly adapted for life underground. Their taxonomy needs a modern revision including chromosomal data as well as morphology. Mole rats of the family Spalacidae range over Turkey and approximately 30 karyotypes of Nannospalax complex inhabit. The diploid number of chromosome of Nannospalax ranges from 36 to 62. Also, fundamental number of chromosomal arms, NF values vary from 66 to 92 while the fundamental number of autosomal arms, NFa ranging from 62 to 88. Unfortunely, karyological studies of the Nannospalax populations on the territory of Turkey on the whole are far from being satisfactory. Karyological studies of this group may yield further chromosomal forms in Turkey, which has a wide range of climatic and biotic conditions and the boundaries of the distribution region of the known species might be determined. In this study, chromosomal forms of Nannospalax in East Anatolia (Erzurum, Kars and Ağrı province) were investigated. The materials of 16 specimens of Nannospalax were collected at 8 different localities in the region. Preparations of mitotic chromosomes were obtained from bone marrows by means of the general air-drying technique. Skins and skulls of specimens have been deposited at the Dicle University, Science Faculty Biology Department. We have identified two chromosomal forms of Nannospalax in East Anatolia of which have diploid chromosome numbers (2n) are 2n = 48 and 2n = 50Öğe A comparative morphological and karyological study on hedgehogs, erinaceus concolor and hemiechinus auritus (insectivora: mammalia) in Diyarbakir province(Kahramanmaraş Sütçü İmam Üniversitesi, 2012-02-09) Ulutürk, Servet; Coşkun, YükselIn this study, we examined the morphological and karyological characteristics of Hemiechinus auritus and Erinaceus concolor (4 male, 3 female) in Diyarbakır province. The karyotypes of E. concolor (2n = 48) and H. auritus (2n = 48) were found similar and but distinctly all the autosomal chromosomes of both species were determined to be biarmed in contrast to the some previously published accounts. There are obvious differences on the tooth roots that could be used in key to genera between Hemiechinus and Erinaceus are determined by the results of this study.Öğe The evaluation of total phenolic, flavonoid, sugar contents and antioxidant activity of tayfi grape in Turkey(Batman Üniversitesi, 2017) Kuru, İbrahim Selçuk; Karakuş Orcan, Pınar; Akbaş, Filiz; Işıkalan, Çiğdem; Namlı, SüreyyaTayfi (Vitis vinifera L.) only grown in the Southeastern Anatolia Region provides a major contribution on the economy of the region people. But, the research related to nutritional content of this species was not found. This study was carried out to determine the total phenolic, flavonoids, sugar content and antioxidant activity of different organe (mature/young seed and leaf ) ‘Tayfi’ grape. The highest amount of total phenolic (380,94 μg GAE/mg extract) was found to be in the young seeds. The high amount of total flavonoid content (107,21 µg QEs/mg extract) was obtained from young leaves. Higher antioxidant activity was observed in young seeds (91,32%) as expected from high contents of total phenolics. In general, as the total phenolics in seed increased, the antioxidant activities also increased. Also, changes in sugar content (glucose, fructose, sucrose and maltose) were determined in leaf from seed (young and mature) of Tayfi grape by HPLC. The highest concentration of sucrose was descripted in mature seed (9,29 mg/g).
